catalog number :
MBS705294
products type :
ELISA Kit
products full name :
Rat IL-1 Receptor Like 1, IL1RL1 ELISA Kit
products short name :
interleukin 1 receptor-like 1
products name syn :
Rat IL-1 Receptor Like 1 (IL1RL1) ELISA Kit; DER4; FIT-1; MGC32623; ST2; ST2L; ST2V; T1; growth stimulation-expressed; homolog of mouse growth stimulation-expressed; interleukin 1 receptor-related protein; interleukin 1 receptor-like 1
other names :
interleukin-1 receptor-like 1 isoform Fit-1M; Interleukin-1 receptor-like 1; Fit-1; Fos-responsive gene 1 protein
products gene name :
IL1RL1
other gene names :
Il1rl1; Il1rl1; Fit1
uniprot entry name :
ILRL1_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IL1RL1. No significant cross-reactivity or interference between rat IL1RL1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1RL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1RL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1RL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1RL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001121161.1
ncbi gb acc num :
NM_001127689.1
ncbi mol weight :
64,406 Da
ncbi summary :
may be involved in modulating IL-1 signaling [RGD, Feb 2006]
uniprot summary :
Function: Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function
size4 :
10x96-Strip-Wells