catalog number :
MBS705290
products type :
ELISA Kit
products full name :
Human Aquaporin 4 Antibody, AQP-4 Ab ELISA Kit
products short name :
Aquaporin 4 Antibody, AQP-4 Ab
products name syn :
Human Aquaporin 4 Antibody; AQP-4 Ab ELISA Kit; Aquaporin 4 Antibody; AQP-4 Ab
other names :
aquaporin-4 isoform b; Aquaporin-4; aquaporin-4; WCH4; aquaporin type4; aquaporin 4 isoform delta4; mercurial-insensitive water channel; aquaporin 4; Mercurial-insensitive water channel; MIWC; WCH4
other gene names :
AQP4; AQP4; MIWC; HMIWC2; AQP-4; MIWC
uniprot entry name :
AQP4_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human AQP-4 antibody. No significant cross-reactivity or interference between human AQP-4 antibody and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 1. 56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of human AQP-4 antibody is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AQP-4. Standards and samples are pipetted into the wells with a biotin-conjugated AQP-4 and avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AQP-4 antibody bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_004019.1
ncbi gb acc num :
NM_004028.3
ncbi mol weight :
32,300 Da
ncbi pathways :
Aquaporin-mediated Transport Pathway (187187); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); Passive Transport By Aquaporins Pathway (187188); SIDS Susceptibility Pathways (198901); Spinal Cord Injury Pathway (739007); Transmembrane Transport Of Small Molecules Pathway (106572); Vasopressin Regulates Renal Water Homeostasis Via Aquaporins Pathway (187189); Vasopressin-regulated Water Reabsorption Pathway (143700); Vasopressin-regulated Water Reabsorption Pathway (143631)
ncbi summary :
This gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
size4 :
10x96-Strip-Wells