catalog number :
MBS705286
products type :
ELISA Kit
products full name :
Rat High density lipoprotein cholesterol, HDL-C ELISA Kit
products short name :
[High density lipoprotein cholesterol, HDL-C]
products name syn :
[Rat High density lipoprotein cholesterol; HDL-C ELISA kit; High density lipoprotein cholesterol; HDL-C]
specificity :
This assay has high sensitivity and excellent specificity for detection of rat HDL. No significant cross-reactivity or interference between rat HDL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 78 ng/ml-5000 ng/ml. Sensitivity: 19.5 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HDL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HDL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HDL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HDL bound in the initial step. The color development is stopped and the intensity of the color is measured.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells