catalog number :
MBS705285
products type :
ELISA Kit
products full name :
Pig Procalcitonin, PCT ELISA Kit
products short name :
Procalcitonin, PCT
products name syn :
Pig Procalcitonin; PCT ELISA Kit; Procalcitonin; PCT
other names :
propionate CoA-transferase Pct; Propionate CoA-transferase Pct; propionate CoA-transferase Pct; Propionate CoA-transferase Pct
other gene names :
pct; pct; RLO149_c008140
uniprot entry name :
F7ZLI1_ROSLO
specificity :
This assay has high sensitivity and excellent specificity for detection of pig PCT. No significant cross-reactivity or interference between pig PCT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 39 pg/ml -2500 pg/ml. Sensitivity: The minimum detectable dose of pig PCT is typically less than 9.75 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCT bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
YP_004689804.1
ncbi gb acc num :
NC_015730.1
ncbi mol weight :
69,733 Da
ncbi pathways :
Microbial Metabolism In Diverse Environments Pathway (377011); Propanoate Metabolism Pathway (376987); Propanoate Metabolism Pathway (387); Pyruvate Metabolism Pathway (376982); Pyruvate Metabolism Pathway (373); Styrene Degradation Pathway (376986); Styrene Degradation Pathway (390)
size4 :
10x96-Strip-Wells