ELISA/assay

Mouse hepcidin, Hepc ELISA Kit

MBS705276

48-Strip-Wells

565 USD

ELISA Kit

Mouse hepcidin, Hepc ELISA Kit

hepcidin antimicrobial peptide

Mouse hepcidin (Hepc) ELISA Kit; HEPC; HEPCIDIN; HFE2B; LEAP-1; LEAP1; PLTR; liver-expressed antimicrobial peptide; putative liver tumor regressor; hepcidin antimicrobial peptide

hepcidin; Hepcidin; hepcidin; hepcidin antimicrobial peptide 1; hepcidin antimicrobial peptide

HAMP

Hamp; Hamp; Hepc; Hamp1; Hepc1; Hamp1; Hepc; Hepc1

HEPC_MOUSE

Mouse

83

This assay has high sensitivity and excellent specificity for detection of mouse Hepc. No significant cross-reactivity or interference between mouse Hepc and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml - 50 ng/ml. Sensitivity: The minimum detectable dose of mouse Hepc is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Hepc has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Hepc present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Hepc is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Hepc bound in the initial step. The color development is stopped and the intensity of the color is measured.

14211542

NP_115930.1

NM_032541.1

Q9EQ21

9,352 Da

Iron Homeostasis Pathway (198312)

Function: Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150