ELISA/assay

Human NAD (P) H Dehydrogenase, Quinone 1, NQO1 ELISA Kit

MBS705270

96 Strip Wells

760 USD

ELISA Kit

Human NAD (P) H Dehydrogenase, Quinone 1, NQO1 ELISA Kit

NAD (P) H dehydrogenase, quinone 1

Human NAD (P) H Dehydrogenase; Quinone 1 (NQO1) ELISA Kit; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; DT-diaphorase; NAD (P) H:Quinone acceptor oxidoreductase type 1; NAD (P) H:menadione oxidoreductase 1; NAD (P) H:quinone oxireductase; azoreductase; diaphorase (NADH/NADPH) ; NAD (P) H dehydrogenase; quinone 1

NAD(P)H dehydrogenase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; azoreductase; diaphorase-4; DT-diaphorase; dioxin-inducible 1; menadione reductase; quinone reductase 1; phylloquinone reductase; NAD(P)H:quinone oxireductase; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H dehydrogenase, quinone 1; Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1

NQO1

NQO1; NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; DIA4; NMOR1; DTD; QR1

NQO1_HUMAN

Human

274

This assay has high sensitivity and excellent specificity for detection of Human NQO1. No significant cross-reactivity or interference between Human NQO1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates and cell lysates. Detection Range: 39 pg/ml-2500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 9.75 pg/ml

Sample Volume: 50-100ul

For Sample: For the quantitative determination of human NAD (P) H dehydrogenase, quinone 1 (NQO1) concentrations in serum, plasma, tissue homogenates. PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NQO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NQO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NQO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NQO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4505415

NP_000894.1

NM_000903.2

P15559

30,868 Da

AhR Pathway 755436!!Dopamine Metabolism Pathway 920995!!Estrogen Metabolism Pathway 198898!!Keap1-Nrf2 Pathway 198777!!Metabolism Pathway 477135!!Metabolism Of Amino Acids And Derivatives Pathway 106169!!Oxidative Stress Pathway 198916!!Regulation Of Ornithine Decarboxylase (ODC) Pathway 106176!!Ubiquinone And Other Terpenoid-quinone Biosynthesis Pathway 82939!!Ubiquinone And Other Terpenoid-quinone Biosynthesis Pathway 300

This gene is a member of the NAD(P)H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD). Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Function: The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Catalytic activity: NAD(P)H + a quinone = NAD(P)+ + a hydroquinone. Cofactor: FAD. Enzyme regulation: Inhibited by dicoumarol.

96 Strip Wells

760 USD