Mouse Hepatocyte Growth Factor Receptor, C-MET/HGFR ELISA Kit | MyBioSource MBS705259 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Hepatocyte Growth Factor Receptor, C-MET/HGFR ELISA Kit

catalog :

MBS705259

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS705259

products type :

ELISA Kit

products full name :

Mouse Hepatocyte Growth Factor Receptor, C-MET/HGFR ELISA Kit

products short name :

[met proto-oncogene (hepatocyte growth factor receptor)]

products name syn :

[Mouse Hepatocyte Growth Factor Receptor (C-MET/HGFR) ELISA Kit; AUTS9; HGFR; RCCP2; c-Met; HGF receptor; SF receptor; met proto-oncogene; met proto-oncogene tyrosine kinase; oncogene MET; scatter factor receptor; met proto-oncogene (hepatocyte growth factor receptor)]

other names :

[Hepatocyte growth factor receptor; Hepatocyte growth factor receptor; hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase; met proto-oncogene; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met]

products gene name :

[MET]

other gene names :

[Met; Met; HGF; HGFR; Par4; c-Met; AI838057; HGF receptor; SF receptor]

uniprot entry name :

MET_MOUSE

reactivity :

Mouse

sequence length :

1379

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse C-MET/HGFR. No significant cross-reactivity or interference between mouse C-MET/HGFR and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich (Quantitative). Detection Range: 0.078 ng/ml -5 ng/ml. Sensitivity: 0.02 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C-MET/HGFR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C-MET/HGFR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C-MET/HGFR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C-MET/HGFR bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

125485

ncbi acc num :

P16056.1

uniprot acc num :

P16056

ncbi mol weight :

153,549 Da

ncbi pathways :

Adherens Junction Pathway (83267); Adherens Junction Pathway (481); Alpha6-Beta4 Integrin Signaling Pathway (198373); Axon Guidance Pathway (83262); Axon Guidance Pathway (476); Axon Guidance Pathway (970585); Bacterial Invasion Of Epithelial Cells Pathway (149817); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460)

uniprot summary :

Function: Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells 1 PublicationManual assertion based on experiment in:Ref.4. Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: In its inactive state, the C-terminal tail interacts with the catalytic domain and inhibits the kinase activity. Upon ligand binding, the C-terminal tail is displaced and becomes phosphorylated, thus increasing the kinase activity

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

435

size3 :

96-Strip-Wells

price3 :

625

size4 :

5x96-Strip-Wells

price4 :

2595

size5 :

10x96-Strip-Wells

price5 :

4905

more info or order :

MyBioSource product webpage