catalog number :
MBS705237
products type :
ELISA Kit
products full name :
Mouse Ciliary Neurotrophic Factor, CNTF ELISA Kit
products short name :
ciliary neurotrophic factor
products name syn :
Mouse Ciliary Neurotrophic Factor; CNTF ELISA Kit; HCNTF; ; ciliary neurotrophic factor
other names :
ciliary neurotrophic factor; Ciliary neurotrophic factor; ciliary neurotrophic factor; ciliary neurotrophic factor
products gene name :
CNTF
other gene names :
Cntf; Cntf; AI429687; CNTF
uniprot entry name :
CNTF_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse CNTF. No significant cross-reactivity or interference between Mouse CNTF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml-400 pg/ml. Sensitivity: 1.56 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CNTF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CNTF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CNTF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CNTF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_740756.1
ncbi gb acc num :
NM_170786.2
ncbi mol weight :
22,587 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Delta-Notch Signaling Pathway (198316); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488)
ncbi summary :
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008]
uniprot summary :
Function: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.
size4 :
10x96-Strip-Wells