Human Glucose-6-Phosphate Dehydrogenase, G6PD ELISA Kit | MyBioSource MBS705229 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glucose-6-Phosphate Dehydrogenase, G6PD ELISA Kit

catalog :

MBS705229

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705229

products type :

ELISA Kit

products full name :

Human Glucose-6-Phosphate Dehydrogenase, G6PD ELISA Kit

products short name :

glucose-6-phosphate dehydrogenase

products name syn :

Human Glucose-6-Phosphate Dehydrogenase; G6PD ELISA Kit; G6PD1; glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase; G6PD; glucose-6-phosphate dehydrogenase

other names :

glucose-6-phosphate 1-dehydrogenase isoform a; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate dehydrogenase

products gene name :

G6PD

other gene names :

G6PD; G6PD; G6PD1; G6PD

uniprot entry name :

G6PD_HUMAN

reactivity :

Human

sequence length :

545

specificity :

This assay has high sensitivity and excellent specificity for detection of human G6PD. No significant cross-reactivity or interference between human G6PD and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 mU/ml -400 mU/ml. Sensitivity: The minimum detectable dose of human G6PD is typically less than 1.56 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for G6PD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any G6PD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for G6PD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of G6PD bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

109389365

ncbi acc num :

NP_000393.4

ncbi gb acc num :

NM_000402.4

uniprot acc num :

P11413

ncbi mol weight :

59,257 Da

ncbi pathways :

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Glutathione Metabolism Pathway (82973); Glutathione Metabolism Pathway (198824); Glutathione Metabolism Pathway (343); Glycogen Storage Diseases Pathway (980468); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196)

ncbi summary :

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.1 PublicationManual assertion based on experiment in:Ref.31. Catalytic activity: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.1 PublicationManual assertion based on experiment in:Ref.31

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage