ELISA/assay

Mouse Kidney injury molecule 1, Kim-1 ELISA Kit

MBS705228

96 Strip Wells

895 USD

ELISA Kit

Mouse Kidney injury molecule 1, Kim-1 ELISA Kit

hepatitis A virus cellular receptor 1

Mouse Kidney injury molecule 1; Kim-1 ELISA Kit; HAVCR; HAVCR-1; KIM-1; KIM1; TIM-1; TIM1; TIMD1; T cell immunoglobin domain and mucin domain protein 1; kidney injury molecule 1; hepatitis A virus cellular receptor 1

hepatitis A virus cellular receptor 1 homolog isoform b; Hepatitis A virus cellular receptor 1 homolog; hepatitis A virus cellular receptor 1 homolog; TIMD-1; HAVcr-1; kidney injury molecule 1; t cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; T-cell immunoglobulin and mucin domain containing 1; t cell immunoglobulin and mucin domain-containing protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; TIMD-1; T cell membrane protein 1; T-cell immunoglobulin mucin receptor 1; TIM-1

HAVCR1

Havcr1; Havcr1; Tim1; KIM-1; TIM-1; Timd1; AI503787; Kim1; Tim1; Timd1; HAVcr-1; KIM-1; TIMD-1; TIM-1

HAVR1_MOUSE

Mouse

282

This assay has high sensitivity and excellent specificity for detection of Mouse HAVCR1. No significant cross-reactivity or interference between Mouse HAVCR1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.94 ng/ml-60 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.23 ng/ml

Sample Volume: 50-100ul

Introduction: Kim-1 (kidney injury molecule-1) is a type I transmembrane glycoprotein expressed on dedifferentiated renal proximal tubule epithelial cells undergoing regeneration after toxic or ischemic injury. The extracellular domain of Kim-1 is composed of an immunoglobulin-like domain topping a long mucin-like domain, a structure that points to a possible role in cell adhesion by homology to several known adhesion proteins. Two splice variants (a and b) of the human Kim-1 having identical extracellular domains, differ in their cytoplasmic domains and tissue distributions. In normal kidneys, Kim-1 is undetectable, but this molecule is abundantly expressed in PTC after ischemic and nephrotoxic injury. In mice, Kim-1 is upregulated in polycystic kidney disease especially in regions of the kidney where there is fibrosis. In humans and in rodents, Kim-1 is induced after ischemic or toxic acute tubular necrosis and the cleaved Kim-1 ectodomain is quantified in the urine as a biomarker for acute kidney injury. The early and abundant tubular expression following different types of injury makes Kim-1 a specific marker of tubular cell injury that may be linked to recuperative or damaging mechanisms that direct the process of interstitial damage. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kim-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Kim-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Kim-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Kim-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

262231823

NP_001160103.1

NM_001166631.1

Q5QNS5

33,361 Da

Focal Adhesion Pathway 83067!!Focal Adhesion Pathway 478!!MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Salmonella Infection Pathway 375172!!Salmonella Infection Pathway 375149

Function: May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair 1 PublicationManual assertion based on experiment in:Ref.4

96 Strip Wells

895 USD