ELISA/assay

Mouse B cell activation factor, BAFF ELISA Kit

MBS705222

96 Strip Wells

895 USD

ELISA Kit

Mouse B cell activation factor, BAFF ELISA Kit

tumor necrosis factor (ligand) superfamily, member 13b

Mouse B cell activation factor; BAFF ELISA Kit; BAFF; BLYS; CD257; DTL; TALL-1; TALL1; THANK; TNFSF20; ZTNF4; ApoL related ligand TALL-1; B-cell activating factor; B-lymphocyte stimulator; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolo; tumor necrosis factor (ligand) superfamily; member 13b

tumor necrosis factor ligand superfamily member 13B; Tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor ligand superfamily member 13B; B-cell-activating factor; b cell-activating factor; tumor necrosis factor (ligand) superfamily, member 13b; B-cell-activating factor; BAFF; CD_antigen: CD257Cleaved into the following 2 chains:Tumor necrosis factor ligand superfamily member 13b, membrane form; Tumor necrosis factor ligand superfamily member 13b, soluble form

TNFSF13B

Tnfsf13b; Tnfsf13b; BAFF; BLyS; TALL1; THANK; zTNF4; TALL-1; TNFSF20; D8Ertd387e; Baff

TN13B_MOUSE

Mouse

309

This assay has high sensitivity and excellent specificity for detection of Mouse TNFSF13B. No significant cross-reactivity or interference between Mouse TNFSF13B and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 15.6 pg/ml -1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BAFF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for BAFF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain BAFF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BAFF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

15826858

NP_296371.1

NM_033622.1

Q9WU72

34,192 Da

Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Intestinal Immune Network For IgA Production Pathway 128761!!Intestinal Immune Network For IgA Production Pathway 128670!!NF-kappa B Signaling Pathway 634537!!Rheumatoid Arthritis Pathway 200310!!Rheumatoid Arthritis Pathway 200269!!Spinal Cord Injury Pathway 739004

Function: Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response.1 PublicationManual assertion based on experiment in:Ref.3

96 Strip Wells

895 USD