ELISA/assay

Human Complement fragment 3c, C3c ELISA Kit

MBS705220

96 Strip Wells

760 USD

ELISA Kit

Human Complement fragment 3c, C3c ELISA Kit

Complement fragment 3c, C3c

Human Complement fragment 3c; C3c ELISA Kit; Complement fragment 3c; C3c

complement component c3a, duplicate 3; Complement component C3-2; complement component c3a, duplicate 3; c3.3; complement component c3c; complement component C3-2; complement component c3a, duplicate 3; Complement component C3-2

C3c

c3a.3; c3c; c3c; C3-3; c3-2; unp301; zgc:103610; C3-2

Q3MU73_DANRE

Human

1651

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, cell culture supernates, urine, saliva. Detection Range: 0.625 ng/ml-40 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.156 ng/ml

Sample Volume: 50-100ul

Introduction: Complement component 3, often simply called C3, is a protein of the immune system. It plays a central role in the complement system and contributes to innate immunity. In humans it is encoded on chromosome 19 by a gene called C3. Soluble C3-convertase catalyzes the proteolytic cleavage of C3 into C3a and C3b as part of the classical complement system as well as the mannan-binding lectin pathway. C3a is an anaphylotoxin, and C3b serves as an opsonizing agent. Factor I can cleave C3b into C3c and C3d. C3c is a 206-amino-acid fragment in the alpha chain (672-1663) of C3b. It is generated when C3b is inactivated (iC3b) and its alpha chain is cleaved by complement factor I into C3c (749-954), and C3dg (955-1303) in the presence complement factor H. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3c. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for C3c and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain C3c, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of C3c in the samples is then determined by comparing the O.D. of the samples to the standard curve

82524318

NP_001032313.1

NM_001037236.1

Q3MU73

184,481 Da

Activation Of C3 And C5 Pathway 1004174!!Alternative Complement Activation Pathway 1004173!!Class A/1 (Rhodopsin-like Receptors) Pathway 1003652!!Complement Cascade Pathway 1004167!!G Alpha (i) Signalling Events Pathway 1003683!!GPCR Downstream Signaling Pathway 1003681!!GPCR Ligand Binding Pathway 1003651!!Herpes Simplex Infection Pathway 377867!!Herpes Simplex Infection Pathway 377865!!Immune System Pathway 1004109

96 Strip Wells

760 USD