NARF Antibody | MyBioSource MBS7052177 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

NARF Antibody

catalog :

MBS7052177

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. Positive WB detected in: A549 whole cell lysate,MCF-7 whole cell lysate,Mouse kidney tissue,Mouse skeletal muscle tissue. All lanes: NARF antibody at 3ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52,57,46,45 kDa. Observed band size: 52 kDa.

image 2 :

Immunofluorescent analysis of Hela cells using MBS7052177 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

product information

catalog number :

MBS7052177

products type :

Antibody

products full name :

NARF Antibody

products short name :

[NARF]

products name syn :

[Nuclear prelamin A recognition factor (Iron-only hydrogenase-like protein 2) (IOP2), NARF]

other names :

[nuclear prelamin A recognition factor isoform c; Nuclear prelamin A recognition factor; nuclear prelamin A recognition factor; nuclear prelamin A recognition factor; Iron-only hydrogenase-like protein 2; IOP2]

products gene name :

[NARF]

other gene names :

[NARF; NARF; IOP2; IOP2]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant Human Nuclear prelamin A recognition factor protein (10-41AA). Conjugate: Non-conjugated. Storage Buffer: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Species: Human. Research Area: Neuroscience

products description :

lamin filament, nuclear lamina, nuclear lumen, lamin binding

ncbi gi num :

84452151

ncbi acc num :

NP_001033707.1

ncbi gb acc num :

NM_001038618.2

uniprot acc num :

Q9UHQ1

ncbi summary :

Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]

uniprot summary :

NARF: Several proteins have been found to be prenylated and methylated at their carboxyl-terminal ends. Prenylation was initially believed to be important only for membrane attachment. However, another role for prenylation appears to be its importance in protein-protein interactions. The only nuclear proteins known to be prenylated in mammalian cells are prelamin A- and B-type lamins. Prelamin A is farnesylated and carboxymethylated on the cysteine residue of a carboxyl-terminal CaaX motif. This post-translationally modified cysteine residue is removed from prelamin A when it is endoproteolytically processed into mature lamin A. The protein encoded by this gene binds to the prenylated prelamin A carboxyl-terminal tail domain. It may be a component of a prelamin A endoprotease complex. The encoded protein is located in the nucleus, where it partially colocalizes with the nuclear lamina. It shares limited sequence similarity with iron-only bacterial hydrogenases. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene, including one with a novel exon that is generated by RNA editing. [provided by RefSeq, Jul 2008]. Chromosomal Location of Human Ortholog: 17q25.3. Cellular Component: lamin filament; nuclear lamina; nuclear lumen. Molecular Function: lamin binding

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage