ELISA/assay

Goat Catalase, Cat ELISA Kit

MBS705199

48-Strip-Wells

590 USD

ELISA Kit

Goat Catalase, Cat ELISA Kit

Catalase (CAT)

Goat Catalase (CAT) ELISA kit; Catalase (CAT)

catalase; Catalase; catalase; catalase

CAT

CAT; CAT

CATA_HUMAN

Goat

527

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Detection Range: 3.12 IU/ml-200 IU/ml. Sensitivity: 0.8IU/ml

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CAT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CAT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CAT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4557014

NP_001743.1

NM_001752.3

P04040

59,756 Da

Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Cellular Responses To Stress Pathway (645258); DNA Damage Response (only ATM Dependent) Pathway (198827); Detoxification Of Reactive Oxygen Species Pathway (941400); Folate Metabolism Pathway (198833); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); Glyoxylate And Dicarboxylate Metabolism Pathway (83002)

This gene encodes catalase, a key antioxidant enzyme in the bodies defense against oxidative stress. Catalase is a heme enzyme that is present in the peroxisome of nearly all aerobic cells. Catalase converts the reactive oxygen species hydrogen peroxide to water and oxygen and thereby mitigates the toxic effects of hydrogen peroxide. Oxidative stress is hypothesized to play a role in the development of many chronic or late-onset diseases such as diabetes, asthma, Alzheimer's disease, systemic lupus erythematosus, rheumatoid arthritis, and cancers. Polymorphisms in this gene have been associated with decreases in catalase activity but, to date, acatalasemia is the only disease known to be caused by this gene. [provided by RefSeq, Oct 2009]

Function: Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.1 PublicationManual assertion based on experiment in:Ref.11. Catalytic activity: 2 H2O2 = O2 + 2 H2O.1 PublicationManual assertion based on experiment in:Ref.11. Cofactor: Heme group.

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440