ELISA/assay

Human Interleukin 5, IL-5 ELISA Kit

MBS705198

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Interleukin 5, IL-5 ELISA Kit

[interleukin 5 (colony-stimulating factor, eosinophil)]

[Human Interleukin 5; IL-5 ELISA KIT; EDF; IL-5; TRF; B cell differentiation factor I; T-cell replacing factor; eosinophil differentiation factor; interleukin 5; interleukin-5; interleukin 5 (colony-stimulating factor; eosinophil)]

[interleukin-5; Interleukin-5; interleukin-5; T-cell replacing factor; B-cell differentiation factor I; eosinophil differentiation factor; colony-stimulating factor, eosinophil; interleukin 5; B-cell differentiation factor I; Eosinophil differentiation factor; T-cell replacing factor; TRF]

[IL5]

[IL5; IL5; EDF; TRF; IL-5; IL-5; TRF]

IL5_HUMAN

Human

134

This assay has high sensitivity and excellent specificity for detection of human IL-5. No significant cross-reactivity or interference between human IL-5 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 pg/ml -50 pg/ml. Sensitivity: 0.195 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4504671

NP_000870.1

NM_000879.2

P05113

15,238 Da

Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051)

This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. [provided by RefSeq, Jul 2013]

Function: Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

320

96-Strip-Wells

445

5x96-Strip-Wells

1880

10x96-Strip-Wells

3540