catalog number :
MBS705196
products type :
ELISA Kit
products full name :
Rabbit Interleukin 10, IL-10 ELISA Kit
products short name :
interleukin 10
products name syn :
Rabbit Interleukin 10; IL-10 ELISA Kit; CSIF; IL-10; IL10A; MGC126450; MGC126451; TGIF; cytokine synthesis inhibitory factor; interleukin 10
other names :
Interleukin-10; Interleukin-10; Cytokine synthesis inhibitory factor; CSIF
products gene name :
IL10
other gene names :
IL10; IL-10; CSIF
uniprot entry name :
IL10_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rabbit IL-10. No significant cross-reactivity or interference between rabbit IL-10 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 7.8 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
20,146 Da
uniprot summary :
Function: Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells
size4 :
10x96-Strip-Wells