ELISA/assay

Human Interleukin 15, IL-15 ELISA Kit

MBS705189

48-Strip-Wells

395 USD

ELISA Kit

Human Interleukin 15, IL-15 ELISA Kit

interleukin 15

Human Interleukin 15; IL-15 ELISA KIT; IL-15; MGC9721; ; interleukin 15

interleukin-15 isoform 1 preproprotein; Interleukin-15; interleukin-15; interleukin 15

IL15

IL15; IL15; IL-15; IL-15

IL15_HUMAN

Human

162

This assay has high sensitivity and excellent specificity for detection of Human IL15. No significant cross-reactivity or interference between Human IL15 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

10835153

NP_000576.1

NM_000585.4

P40933

18,086 Da

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response Pathway (198794); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); Intestinal Immune Network For IgA Production Pathway (128760); Intestinal Immune Network For IgA Production Pathway (128670); Jak-STAT Signaling Pathway (83077)

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]

Function: Cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL-15 requires interaction of IL-15 with components of IL-2R, including IL-2R beta and probably IL-2R gamma but not IL-2R alpha.

48-Strip-Wells

395 USD

96-Strip-Wells

550

5x96-Strip-Wells

2145

10x96-Strip-Wells

3995