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ELISA/assay

Human carbonic anhydrase 2, CA-2 ELISA Kit

MBS705181

96 Strip Wells

865 USD

MBS705181

ELISA Kit

Human carbonic anhydrase 2, CA-2 ELISA Kit

carbonic anhydrase II

Human carbonic anhydrase 2; CA-2 ELISA Kit; CA-II; CAII; Car2; carbonate dehydratase II; carbonic anhydrase B; carbonic dehydratase; carbonic anhydrase II

carbonic anhydrase 2 isoform 1; Carbonic anhydrase 2; carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76; carbonic anhydrase II; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II

CA2

CA2; CA2; CAC; CAII; Car2; CA-II; HEL-76; CAC; CA-II

CAH2_HUMAN

Human

260

This assay has high sensitivity and excellent specificity for detection of Human CA2. No significant cross-reactivity or interference between Human CA2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Range: 0.31 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.08 ng/ml

Sample Volume: 50-100ul

Introduction: The carbonic anhydrases form a family of enzymes that catalyze the rapid conversion of carbon dioxide to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are therefore classified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta and epsilon). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Carbonic anhydrase II (gene name CA2), is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of Sodium in the proximal tubule. Among the carbonic anhydrase isoenzymes, CAII has the highest catalytic efficiency (106 reactions/s) and is the isoenzyme that has been studied the most. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CAII and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CAII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CAII in the samples is then determined by comparing the O.D. of the samples to the standard curve.

4557395

NP_000058.1

NM_000067.2

P00918

29,246 Da

Bile Secretion Pathway 193146!!Bile Secretion Pathway 193095!!Collecting Duct Acid Secretion Pathway 147586!!Collecting Duct Acid Secretion Pathway 147560!!Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway 645347!!Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway 645348!!Gastric Acid Secretion Pathway 154409!!Gastric Acid Secretion Pathway 154383!!Metabolism Pathway 477135!!Nitrogen Metabolism Pathway 83025

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

Function: Essential for bone resorption and osteoclast differentiation Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.3 PublicationsManual assertion based on experiment in:Ref.13. Catalytic activity: H2CO3 = CO2 + H2O. Cofactor: Zinc. Can also use cobalt(II) with lower efficiency, but not copper(II), nickel(II) and manganese(II).1 PublicationManual assertion based on experiment in:Ref.105. Enzyme regulation: Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala-His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO3). Esterase activity weakly reduced by cyanamide. N-hydroxyurea interfers with zinc binding and inhibit activity.27 PublicationsManual assertion based on experiment in:Ref.14

96 Strip Wells

865 USD