ELISA/assay

Human connective tissue growth factor, CTGF ELISA Kit

MBS705169

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human connective tissue growth factor, CTGF ELISA Kit

[connective tissue growth factor]

[Human connective tissue growth factor (CTGF) ELISA kit; CCN2; HCS24; IGFBP8; MGC102839; NOV2; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8; connective tissue growth factor]

[connective tissue growth factor; Connective tissue growth factor; connective tissue growth factor; CCN family member 2; connective tissue growth factor<; CCN family member 2]

[CTGF]

[CTGF; CTGF; CCN2]

CTGF_BOVIN

Human

349

This assay has high sensitivity and excellent specificity for detection of mouse CTGF. No significant cross-reactivity or interference between mouse CTGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernates, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.78 pg/ml-50 pg/ml. Sensitivity: 0.195 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CTGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

27806677

NP_776455.1

NM_174030.2

O18739

37,924 Da

Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (1009296); Gene Expression Pathway (942919); Generic Transcription Pathway (1009207); Hippo Signaling Pathway (749780); Hippo Signaling Pathway (750388); Metabolism Pathway (942801); Metabolism Of Lipids And Lipoproteins Pathway (942902); PPARA Activates Gene Expression Pathway (1009311); Regulation Of Lipid Metabolism By Peroxisome Proliferator-activated Receptor Alpha (PPARalpha) Pathway (1009310); YAP1- And WWTR1 (TAZ)-stimulated Gene Expression Pathway (1009210)

Function: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells Enhances fibroblast growth factor-induced DNA synthesis

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465