ELISA/assay

Human beta-galactosidase, betaGAL ELISA Kit

MBS705153

48-Strip-Wells

565 USD

ELISA Kit

Human beta-galactosidase, betaGAL ELISA Kit

galactosidase, beta 1

Human beta-galactosidase; betaGAL ELISA Kit; EBP; ELNR1; elastin receptor 1; 67kDa; galactosidase; beta 1

beta-galactosidase isoform a preproprotein; Beta-galactosidase; beta-galactosidase; lactase; acid beta-galactosidase; elastin receptor 1, 67kDa; galactosidase, beta 1; Acid beta-galactosidase; Lactase; Elastin receptor 1

GLB1

GLB1; GLB1; EBP; ELNR1; MPS4B; ELNR1; Lactase

BGAL_HUMAN

Human

677

This assay has high sensitivity and excellent specificity for detection of human betaGAL. No significant cross-reactivity or interference between human betaGAL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ulU/ml-500 ulU/ml. Sensitivity: The minimum detectable dose of human betaGAL is typically less than 1.95 ulU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for betaGAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any betaGAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for betaGAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of betaGAL bound in the initial step. The color development is stopped and the intensity of the color is measured.

119372308

NP_000395.2

NM_000404.2

P16278

76,075 Da

Asparagine N-linked Glycosylation Pathway (161013); Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway (161014); Disease Pathway (530764); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (645297); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (82997)

This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]

Function: Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150