catalog number :
MBS705149
products type :
ELISA Kit
products full name :
Human mitogen activited protein kinase, MAPK ELISA Kit
products short name :
mitogen activited protein kinase, MAPK
products name syn :
Human mitogen activited protein kinase; MAPK ELISA Kit; mitogen activited protein kinase; MAPK
other names :
MAPK; MAPK; MAPK
products gene name :
MAPK
uniprot entry name :
B1N8Y5_9ROSA
specificity :
This assay has high sensitivity and excellent specificity for detection of human MAPK. No significant cross-reactivity or interference between human MAPK and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 83 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human MAPK is typically less than 33 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest human MAPK concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<10%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for MAPK. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
ABR10070.1
ncbi mol weight :
42,949 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells