ELISA/assay

Human Cathepsin S, CTSS ELISA Kit

MBS705148

96 Strip Wells

670 USD

ELISA Kit

Human Cathepsin S, CTSS ELISA Kit

cathepsin S

Human Cathepsin S (CTSS) ELISA Kit; MGC3886; ; cathepsin S

cathepsin S isoform 2 preproprotein; Cathepsin S; cathepsin S; cathepsin S

CTSS

CTSS; CTSS

CATS_HUMAN

Human

281

This assay has high sensitivity and excellent specificity for detection of Human CTSS. No significant cross-reactivity or interference between Human CTSS and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Detection Range: 1.25 ng/ml-80 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.31 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CTSS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CTSS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CTSS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CTSS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

315075311

NP_001186668.1

NM_001199739.1

P25774

37,496 Da

Adaptive Immune System Pathway 366160!!Antigen Processing And Presentation Pathway 83074!!Antigen Processing And Presentation Pathway 485!!Antigen Processing-Cross Presentation Pathway 477122!!Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway 730306!!Class I MHC Mediated Antigen Processing Presentation Pathway 366161!!Collagen Formation Pathway 645288!!Degradation Of The Extracellular Matrix Pathway 576263!!Endosomal/Vacuolar Pathway 477125!!Extracellular Matrix Organization Pathway 576262

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Function: Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N. Catalytic activity: Similar to cathepsin L, but with much less activity on Z-Phe-Arg- -NHMec, and more activity on the Z-Val-Val-Arg- -Xaa compound.

96 Strip Wells

670 USD