Mouse High mobility group protein B1, HMGB-1 ELISA Kit | MyBioSource MBS705143 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse High mobility group protein B1, HMGB-1 ELISA Kit

catalog :

MBS705143

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705143

products type :

ELISA Kit

products full name :

Mouse High mobility group protein B1, HMGB-1 ELISA Kit

products short name :

high-mobility group box 1

products name syn :

Mouse High mobility group protein B1; HMGB-1 ELISA Kit; DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot; high-mobility group box 1

other names :

high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; high mobility group protein 1; high mobility group box 1; High mobility group protein 1; HMG-1

products gene name :

HMGB1

other gene names :

Hmgb1; Hmgb1; DEF; p30; Hmg1; HMG-1; SBP-1; amphoterin; Hmg-1; Hmg1; HMG-1

uniprot entry name :

HMGB1_MOUSE

reactivity :

Mouse

sequence length :

215

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse HMGB1. No significant cross-reactivity or interference between Mouse HMGB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMGB-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGB-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6754208

ncbi acc num :

NP_034569.1

ncbi gb acc num :

NM_010439.3

uniprot acc num :

P63158

ncbi mol weight :

24,894 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (970703); Activation Of DNA Fragmentation Factor Pathway (1000455); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1001253); Apoptosis Pathway (970512); Apoptosis Induced DNA Fragmentation Pathway (1000454); Apoptotic Execution Phase Pathway (1000449); Base Excision Repair Pathway (83240); Base Excision Repair Pathway (451); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1001263); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1001271)

ncbi summary :

This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]

uniprot summary :

Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.1 PublicationManual assertion based on experiment in:Ref.7

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage