catalog number :
MBS705139
products type :
ELISA Kit
products full name :
Rat angiotensin II, ANG-II ELISA Kit
products short name :
angiotensin II
products name syn :
Rat angiotensin II (ANG-II) ELISA kit; angiotensin II
products gene name :
ANG-II
specificity :
This assay has high sensitivity and excellent specificity for detection of rat ANG-II. No significant cross-reactivity or interference between rat ANG-II and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of rat angiotensin II (ANG-II) concentrations in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Detection Range: 4.7 pg/ml -300 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of rat ANG-II is typically less than 1.17 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANG-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANG-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANG-II is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANG-II bound in the initial step. The color development is stopped and the intensity of the color is measured.