ELISA/assay

Human Ghrelin, GHRL ELISA Kit

MBS705124

96 Strip Wells

850 USD

ELISA Kit

Human Ghrelin, GHRL ELISA Kit

ghrelin/obestatin prepropeptide

Human Ghrelin (GHRL) ELISA Kit; MTLRP; obestatin; OTTHUMP00000207794; OTTHUMP00000207795; OTTHUMP00000207796; OTTHUMP00000207798; ghrelin; growth hormone secretagogue receptor ligand; ghrelin/obestatin; ghrelin/obestatin preprohormone; m; ghrelin/obestatin prepropeptide

appetite-regulating hormone isoform 2 preproprotein; Appetite-regulating hormone; appetite-regulating hormone; motilin-related peptide; growth hormone secretagogue; ghrelin/obestatin preprohormone; growth hormone-releasing peptide; prepro-appetite regulatory hormone; ghrelin, growth hormone secretagogue receptor ligand; ghrelin/obestatin prepropeptide; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related peptide; Protein M46Cleaved into the following 3 chains:Ghrelin-27; Ghrelin-28; Ghrelin; Obestatin

GHRL

GHRL; GHRL; MTLRP; MTLRP; UNQ524/PRO1066; Ghrelin

GHRL_HUMAN

Human

116

This assay has high sensitivity and excellent specificity for detection of Human GHRL. No significant cross-reactivity or interference between Human GHRL and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.625 pg/ml-40 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.156 pg/ml

Sample Volume: 50-100ul

Introduction: Ghrelin, the name is based on its role as a growth hormone-releasing peptide, is a hormone produced mainly by P/D1 cells lining the fundus of the human stomach and epsilon cells of the pancreas that stimulates hunger. Ghrelin levels increase before meals and decrease after meals. It is considered the counterpart of the hormone leptin, produced by adipose tissue, which induces satiation when present at higher levels. In some bariatric procedures, the level of ghrelin is reduced in patients, thus causing satiation before it would normally occur. Ghrelin is also produced in the hypothalamic arcuate nucleus, where it stimulates the secretion of growth hormone from the anterior pituitary gland. Receptors for ghrelin are expressed by neurons in the arcuate nucleus and the ventromedial hypothalamus. The ghrelin receptor is a G protein-coupled receptor, formerly known as the GHS receptor (growth hormone secretagogue receptor). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GHRL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for GHRL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain GHRL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of GHRL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

201860279

NP_001128413.1

NM_001134941.1

Q9UBU3

12,911 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Metabolism Of Proteins Pathway 106230!!Peptide Hormone Metabolism Pathway 771603!!Peptide Ligand-binding Receptors Pathway 106358!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356

This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]

Function: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.

96 Strip Wells

850 USD