ELISA/assay

Rat Corticosterone, CORT ELISA Kit

MBS705109

96 Strip Wells

915 USD

ELISA Kit

Rat Corticosterone, CORT ELISA Kit

Corticosterone, CORT

Rat Corticosterone; CORT ELISA Kit; Corticosterone; CORT

cortistatin preproprotein; Cortistatin; cortistatin; cortistatin-14; cortistatin-17; cortistatin-29; preprocortistatin; prepro-cortistatin; cortistatin

CORT

CORT; CORT; CST-14; CST-17; CST-29; UNQ307/PRO350

CORT_HUMAN

Rat

105

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.2 ng/ml-40 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.1 ng/ml

Sample Volume: 50-100ul

Introduction: Corticosterone is a 21 carbon glucocorticoid produced by the adrenal cortex in response to ACTH (adrenocoridotropic hormone), and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress. Biologists often measure corticosterone in fecal material of the species they are studying. This non-invasive sample collection procedure has the advantage of not itself causing stress, and thereby increasing corticosterone levels, a common problem where samples for measurement of corticosterone are collected by more invasive means. Plasma corticosterone levels have a circadian variation, and may be important in the regulation of the sleep-wake cycle. Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for CORT and Horseradish Peroxidase (HRP) conjugated CORT, then incubated. A competitive inhibition reaction is launched between CORT (Standards or samples) and Horseradish Peroxidase (HRP) conjugated CORT with the antibody specific for CORT. The more the amount of CORT in samples, the less antibody bound by Horseradish Peroxidase (HRP) conjugated CORT. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of CORT in the sample. The color development is stopped and the intensity of the color is measured.

311893369

NP_001293.3

NM_001302.4

O00230

11,532 Da

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!M Phase Pathway 105812!!Mitotic Anaphase Pathway 105818!!Mitotic Metaphase And Anaphase Pathway 730317!!Mitotic Prometaphase Pathway 105815!!Resolution Of Sister Chromatid Cohesion Pathway 730315!!Separation Of Sister Chromatids Pathway 730318

This gene encodes a neuropeptide that is structurally similar to somatostatin. It binds to all known somatostatin receptors, and shares many pharmacological and functional properties with somatostatin, including the depression of neuronal activity. However, it also has many properties distinct from somatostatin, such as induction of slow-wave sleep, apparently by antagonism of the excitatory effects of acetylcholine on the cortex, reduction of locomotor activity, and activation of cation selective currents not responsive to somatostatin. The preproprotein undergoes further processing into multiple mature products. Read-through transcripts exist between this gene and the upstream APITD1 (apoptosis-inducing, TAF9-like domain 1) gene, as represented in GeneID:100526739. [provided by RefSeq, Nov 2010]

Function: Binds to all human somatostatin receptor (SSTR) subtypes. It also inhibits cAMP production induced by forskolin through SSTRs.

96 Strip Wells

915 USD