catalog number :
MBS705091
products type :
ELISA Kit
products full name :
Rat Elastase ELISA Kit
products short name :
Elastase
products name syn :
Rat Elastase ELISA Kit; Elastase
other names :
elastase; Elastase; Elastase
uniprot entry name :
Q25510_MANSE
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Elastase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Elastase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Elastase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Elastase bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAA67842.1
ncbi mol weight :
31,271 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells