GRHL3 Antibody | MyBioSource MBS7050879 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GRHL3 Antibody

catalog :

MBS7050879

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. Positive WB detected in:A549 whole cell lysate,HepG2 whole cell lysate,Mouse heart tissue. All lanes: GRHL3 antibody at 2.8ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 71,69,63,58,68 kDa. Observed band size: 71,69 kDa.

image 2 :

Immunofluorescent analysis of PC3 cells using MBS7050879 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

product information

catalog number :

MBS7050879

products type :

Antibody

products full name :

GRHL3 Antibody

products short name :

[GRHL3]

products name syn :

[Grainyhead-like protein 3 homolog; Sister of mammalian grainyhead; Transcription factor CP2-like 4; GRHL3]

other names :

[grainyhead-like protein 3 homolog isoform 4; Grainyhead-like protein 3 homolog; grainyhead-like protein 3 homolog; grainyhead like transcription factor 3; Sister of mammalian grainyhead; Transcription factor CP2-like 4]

products gene name :

[GRHL3]

other gene names :

[GRHL3; GRHL3; SOM; VWS2; TFCP2L4]

uniprot entry name :

GRHL3_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

556

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

tested application :

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

app notes :

WB: 1: 500-1: 5000. IF: 1: 50-1: 200

image1 heading :

Western Blot (WB)

image2 heading :

Immunofluorescence (IF)

other info1 :

Immunogen: Recombinant Human Grainyhead-like protein 3 homolog protein (31-182AA)

other info2 :

Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Conjugation: Non-conjugated. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-101968 / sc-131537 / sc-131538

products description :

Transcription factor playing important roles in primary neurulation and in the differentiation of stratified epithelia of both ectodermal and endodermal origin (By similarity). Binds directly to the consensus DNA sequence 5'-AACCGGTT-3' acting as an activator and repressor on distinct target genes (PubMed: 21081122, PubMed: 25347468). xhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair (By similarity). Exhibits functional redundancy with GRHL2 in epidermal morphogenetic events and epidermal wound repair but is essential to form the epidermal barrier with TGM3 as critical direct target gene among others. Despite being dispensable during normal epidermal homeostasis in the adulthood, is again required for barrier repair after immune-mediated epidermal damage, regulates distinct gene batteries in embryonic epidermal differentiation and adult epidermal barrier reformation after injury. Plays unique and cooperative roles with GRHL2 in establishing distinct zones of primary neurulation. Essential for spinal closure, functions cooperatively with GRHL2 in closure 2 (forebrain/midbrain boundary) and posterior neuropore closure (By similarity). Also required for proper development of the oral periderm (PubMed: 24360809). No genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity (PubMed: 21081122).

ncbi gi num :

303324555

ncbi acc num :

NP_001181939.1

ncbi gb acc num :

NM_001195010.1

uniprot acc num :

Q8TE85

ncbi mol weight :

67,696 Da

ncbi summary :

This gene encodes a member of the grainyhead family of transcription factors. The encoded protein may function as a transcription factor during development, and has been shown to stimulate migration of endothelial cells. Multiple transcript variants encoding distinct isoforms have been identified for this gene.[provided by RefSeq, Aug 2010]

uniprot summary :

GRHL3: May function as a transcription factor. Belongs to the grh/CP2 family. Grainyhead subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: cytoplasm; nucleoplasm; nucleus. Molecular Function: chromatin DNA binding; protein binding; sequence-specific DNA binding. Biological Process: epidermis development; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; regulation of actin cytoskeleton organization and biogenesis. Disease: Van Der Woude Syndrome 2

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage