ELISA/assay

Human terminal complement complex SC5b9 ELISA Kit

MBS705084

48-Strip-Wells

565 USD

ELISA Kit

Human terminal complement complex SC5b9 ELISA Kit

terminal complement complex SC5b9

Human terminal complement complex SC5b9 ELISA kit; terminal complement complex SC5b9

Human

This assay has high sensitivity and excellent specificity for detection of human SC5b9. No significant cross-reactivity or interference between human SC5b9 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 ng/ml -2000 ng/ml. Sensitivity: 7.81 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SC5b9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SC5b9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SC5b9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SC5b9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150