Human malonyl coenzyme A ELISA Kit | MyBioSource MBS705079 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human malonyl coenzyme A ELISA Kit

catalog :

MBS705079

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705079

products type :

ELISA Kit

products full name :

Human malonyl coenzyme A ELISA Kit

products short name :

malonyl coenzyme A

products name syn :

Human malonyl coenzyme A ELISA Kit; malonyl coenzyme A

other names :

malonyl-CoA decarboxylase, mitochondrial; Malonyl-CoA decarboxylase, mitochondrial; malonyl-CoA decarboxylase, mitochondrial; malonyl coenzyme A decarboxylase; malonyl-CoA decarboxylase

products gene name :

malonyl CoA

other gene names :

MLYCD; MLYCD; MCD; MCD

uniprot entry name :

DCMC_HUMAN

reactivity :

Human

sequence length :

493

specificity :

This assay has high sensitivity and excellent specificity for detection of human malonyl CoA. No significant cross-reactivity or interference between human malonyl CoA and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.156 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for malonyl CoA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any malonyl CoA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for malonyl CoA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of malonyl CoA bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

110349750

ncbi acc num :

NP_036345.2

ncbi gb acc num :

NM_012213.2

uniprot acc num :

O95822

ncbi mol weight :

50,946 Da

ncbi pathways :

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Peroxisomal Lipid Metabolism Pathway (106136); Peroxisome Pathway (131226); Peroxisome Pathway (131126); Propanoate Metabolism Pathway (83004); Propanoate Metabolism Pathway (387)

ncbi summary :

The product of this gene catalyzes the breakdown of malonyl-CoA to acetyl-CoA and carbon dioxide. Malonyl-CoA is an intermediate in fatty acid biosynthesis, and also inhibits the transport of fatty acyl CoAs into mitochondria. Consequently, the encoded protein acts to increase the rate of fatty acid oxidation. It is found in mitochondria, peroxisomes, and the cytoplasm. Mutations in this gene result in malonyl-CoA decarboyxlase deficiency. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids. Plays a role in the metabolic balance between glucose and lipid oxidation in muscle independent of alterations in insulin signaling. May play a role in controlling the extent of ischemic injury by promoting glucose oxidation.4 PublicationsManual assertion based on experiment in:Ref.2. Catalytic activity: Malonyl-CoA = acetyl-CoA + CO2.5 PublicationsManual assertion based on experiment in:Ref.1. Enzyme regulation: Malonyl-CoA decarboxylase activity does not require any cofactors or divalent metal ions. Formation of interchain disulfide bonds leads to positive cooperativity between active sites and increases the affinity for malonyl-CoA and the catalytic efficiency (in vitro).2 PublicationsManual assertion based on experiment in:Ref.6

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage