ELISA/assay

Rat Obestatin ELISA Kit

MBS705074

96 Strip Wells

850 USD

ELISA Kit

Rat Obestatin ELISA Kit

Obestatin

Rat Obestatin ELISA Kit; Obestatin

Rat

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 7.8pg/ml-500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 1.95pg/ml

Sample Volume: 50-100ul

Introduction: Obestatin is a 23 amino acid residues peptide isolated from the rat stomach.The peptide shares the precursor with a food intake stimulating peptide, ghrelin,but possesses reducing effects on food intake, gut motility and body weight.With the use of an antiserum directed against the mouse/rat obestatin, obestatin immunoreactivity (irOBS) was detected in cells of the gastric mucosa and myenteric plexus and in Leydig cells of the testis in Sprague-Dawley rats. Double labeling of myenteric plexus with antisera against obestatin and choline acetyltransferase (ChAT) revealed that nearly all irOBS neurons were ChAT positive and vice versa.Obestatin (100nM) added to dissociated and cultured rat cerebral cortical neurons elevated cytosolic calcium concentrations [Ca+2]i in a population of cortical neurons.Intracerebroventricular admini- stration of obestatin inhibited water drinking in ad libitum fed and watered rats, and in food and water deprived animals. In addition, obestatin inhibited angiotensin II-induced water drinking in animals provided free access to water and food.Obestatin peptides had no effect on insulin sensitivity as revealed by hypoglycaemic response when co-administered with insulin, supporting a role of obestatin in regulating metabolism through changes of appetite,but indicating no direct actions on glucose homeostasis or insulin secretion.It is supposed that in rats the effects of obestatin on food intake may be secondary to an action of the peptide to inhibit water drinking. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Obestatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Obestatin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Obestatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Obestatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD