ELISA/assay

Rat Osteocalcin/Bone gla protein, OT/BGP ELISA Kit

MBS705066

96 Strip Wells

915 USD

ELISA Kit

Rat Osteocalcin/Bone gla protein, OT/BGP ELISA Kit

bone gamma-carboxyglutamate (gla) protein

Rat Osteocalcin/Bone gla protein; OT/BGP ELISA kit; RP11-54H19.5; BGP; OC; PMF1; bone Gla protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin) ; gamma-carboxyglutamic acid-containing protein; osteocalcin; bone gamma-carboxyglutamate (gla) protein

Osteocalcin; Osteocalcin; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein

BGLAP

BGLAP; BGP

OSTCN_RABIT

Rat

49

This assay has high sensitivity and excellent specificity for detection of Rat BGLAP. No significant cross-reactivity or interference between Rat BGLAP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates, tissue homogenates, urine. Detection Range: 0.312 pg/ml-20 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 3: Biomineralization

Introduction: Osteocalcin is a noncollagenous protein found in bone and dentin. It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis. It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown. As osteocalcin is manufactured by osteoblasts, it is often used as a biochemical marker, or biomarker, for the bone formation process. It has been routinely observed that higher serum-osteocalcin levels are relatively well correlated with increases in bone mineral density (BMD) during treatment with anabolic bone formation drugs for osteoporosis, such as Forteo. In many studies, Osteocalcin is used as a preliminary biomarker on the effectiveness of a given drug on bone formation. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for OT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

730242

P39056.1

P39056

5,431 Da

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!DNA Replication Pathway 105897!!FGF Signaling Pathway 137989!!Glucocorticoid Receptor Regulatory Network Pathway 138014!!M Phase Pathway 105812!!Mitotic M-M/G1 Phases Pathway 160943!!Mitotic Prometaphase Pathway 105815!!Notch-mediated HES/HEY Network Pathway 169347!!Regulation Of Retinoblastoma Protein Pathway 137916

Function: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

96 Strip Wells

915 USD