catalog number :
MBS705061
products type :
ELISA Kit
products full name :
Rat Interleukin 13, IL-13 ELISA Kit
products short name :
[interleukin 13]
products name syn :
[Rat Interleukin 13; IL-13 ELISA KIT; ALRH; BHR1; IL-13; MGC116786; MGC116788; MGC116789; P600; ; interleukin 13]
other names :
[interleukin-13; Interleukin-13; interleukin-13; IL-13; T-cell activation protein P600; interleukin 13; T-cell activation protein P600]
products gene name :
[IL13]
other gene names :
[Il13; Il13; Il-13; IL-13]
uniprot entry name :
IL13_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IL-13. No significant cross-reactivity or interference between rat IL-13 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_446280.1
ncbi gb acc num :
NM_053828.1
ncbi mol weight :
14,093 Da
ncbi pathways :
Asthma Pathway (83508); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521); Fc Epsilon RI Signaling Pathway (83474); Fc Epsilon RI Signaling Pathway (493); Inflammatory Bowel Disease (IBD) Pathway (842782); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83469)
ncbi summary :
cytokine; involved in inflammatory and immune responses [RGD, Feb 2006]
uniprot summary :
Function: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells