catalog number :
MBS705059
products type :
ELISA Kit
products full name :
Mouse Macrophage Inflammatory Protein 2, MIP-2 ELISA Kit
products short name :
chemokine (C-X-C motif) ligand 2
products name syn :
Mouse Macrophage Inflammatory Protein 2; MIP-2 ELISA kit; CINC-2a; GRO2; GROb; MGSA-b; MIP-2a; MIP2; MIP2A; SCYB2; GRO2 oncogene; MGSA beta; melanoma growth stimulatory activity beta; chemokine (C-X-C motif) ligand 2
other names :
C-X-C motif chemokine 2; C-X-C motif chemokine 2; C-X-C motif chemokine 2; macrophage inflammatory protein 2; small inducible cytokine subfamily, member 2; chemokine (C-X-C motif) ligand 2; Macrophage inflammatory protein 2; MIP2
products gene name :
CXCL2
other gene names :
Cxcl2; Cxcl2; GROb; Gro2; Mip2; Scyb; MIP-2; Scyb2; MIP-2a; Mgsa-b; CINC-2a; Mip-2; Mip2; Scyb2; MIP2
uniprot entry name :
CXCL2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse CXCL2. No significant cross-reactivity or interference between Mouse CXCL2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell culture supernates. Detection Range: 12.5 pg/ml-800 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.12 pg/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 2: chemokine. Protein Biological Process 3: Chemotaxis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIP-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIP-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIP-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIP-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033166.1
ncbi gb acc num :
NM_009140.2
ncbi mol weight :
10,621 Da
ncbi pathways :
Chemokine Receptors Bind Chemokines Pathway 1000944!!Chemokine Signaling Pathway 99271!!Chemokine Signaling Pathway 672435!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 1000942!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!G Alpha (i) Signalling Events Pathway 1000973!!GPCR Downstream Signaling Pathway 1000971!!GPCR Ligand Binding Pathway 1000941
uniprot summary :
Function: Chemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst.