catalog number :
MBS705057
products type :
ELISA Kit
products full name :
Mouse Free Tri-iodothyronine Indes, Free-T3 ELISA Kit
products short name :
Free Tri-iodothyronine Indes, Free-T3
products name syn :
Mouse Free Tri-iodothyronine Indes; Free-T3 ELISA Kit; Mouse Free Tri-iodothyronine Indes; Free-T3
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Competitive. Detection Range: 2pmol/l-32pmol/l. Sensitivity: 0.38pmol/L
other info2 :
Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Thyroid function
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T3. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated T3. A competitive inhibition reaction is launched between T3 (Standards or samples) and Biotin-conjugated T3 with the pre-coated antibody specific for T3. The more amount of T3 in samples, the less antibody bound by Biotin-conjugated T3. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of Free-T3 in the sample. The color development is stopped and the intensity of the color is measured.
size3 :
10x96-Strip-Wells