catalog number :
MBS705051
products type :
ELISA Kit
products full name :
Mouse beta2-microglobulin, BMG/beta2-MG ELISA Kit
products short name :
beta-2-microglobulin
products name syn :
Mouse beta2-microglobulin; BMG/beta2-MG ELISA Kit; beta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
other names :
beta-2-microglobulin; Beta-2-microglobulin; beta-2-microglobulin; beta-2 microglobulin
other gene names :
B2m; B2m; Ly-m11; beta2m; beta2-m
uniprot entry name :
B2MG_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse beta2-MG. No significant cross-reactivity or interference between mouse beta2-MG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Competitive. Detection Range: 12.5 ng/ml-800 ng/ml. Sensitivity: 3.12 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for beta2-MG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a biotin-conjugated beta2-MG. A competitive inhibition reaction is launched between beta2-MG (Standards or samples) and biotin-conjugated beta2-MG with the pre-coated antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of beta2-MG in sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033865.2
ncbi gb acc num :
NM_009735.3
ncbi mol weight :
13,779 Da
ncbi pathways :
Adaptive Immune System Pathway (971210); Amyloids Pathway (1000612); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (1001198); Antigen Processing And Presentation Pathway (83271); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (1001199); Class I MHC Mediated Antigen Processing Presentation Pathway (1001196); Cytokine Signaling In Immune System Pathway (970814); DAP12 Interactions Pathway (971203); DAP12 Signaling Pathway (971214)
uniprot summary :
Function: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.
size4 :
10x96-Strip-Wells