product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat leukemia inhibitory factor, LIF ELISA Kit
catalog :
MBS705048
quantity :
48-Strip-Wells
price :
605 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS705048
products type :
ELISA Kit
products full name :
Rat leukemia inhibitory factor, LIF ELISA Kit
products short name :
leukemia inhibitory factor (cholinergic differentiation factor)
products name syn :
Rat leukemia inhibitory factor; LIF ELISA Kit; CDF; DIA; HILDA; D factor; cholinergic differentiation factor; differentiation inhibitory activity; differentiation stimulating factor; leukemia inhibitory factor (cholinergic differentiation factor)
other names :
Leukemia inhibitory factor; Leukemia inhibitory factor; leukemia inhibitory factor; cholinergic neuronal differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; Cholinergic neuronal differentiation factor
products gene name :
LIF
other gene names :
Lif; Lif; LIF
uniprot entry name :
LIF_RAT
reactivity :
Rat
sequence length :
202
specificity :
This assay has high sensitivity and excellent specificity for detection of rat LIF. No significant cross-reactivity or interference between rat LIF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, bronchoalveolar lavage fluid, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of rat LIF is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LIF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
126281
ncbi acc num :
P17777.1
uniprot acc num :
P17777
ncbi mol weight :
22,113 Da
ncbi pathways :
Adipogenesis Pathway (198479); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488); TGF Beta Signaling Pathway (198444); TNF Signaling Pathway (812267); TNF Signaling Pathway (813210)
ncbi summary :
a secreted cytokine; involved in embryonic stem cell and myeloid cell growth and differentiation and stimulation of bone remodeling [RGD, Feb 2006]
uniprot summary :
Function: LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
size1 :
48-Strip-Wells
price1 :
605 USD
size2 :
96-Strip-Wells
price2 :
870
size3 :
5x96-Strip-Wells
price3 :
2950
size4 :
10x96-Strip-Wells
price4 :
5535
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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