ELISA/assay

Human Peroxiredoxin 4, PRDX4 ELISA Kit

MBS705045

48-Strip-Wells

565 USD

ELISA Kit

Human Peroxiredoxin 4, PRDX4 ELISA Kit

peroxiredoxin 4

Human Peroxiredoxin 4 (PRDX4) ELISA Kit; AOE37-2; PRX-4; thioredoxin peroxidase (antioxidant enzyme) ; peroxiredoxin 4

peroxiredoxin-4; Peroxiredoxin-4; peroxiredoxin-4; prx-IV; peroxiredoxin IV; antioxidant enzyme AOE372; thioredoxin peroxidase AO372; thioredoxin peroxidase (antioxidant enzyme); thioredoxin-dependent peroxide reductase A0372; epididymis secretory sperm binding protein Li 97n; peroxiredoxin 4; Antioxidant enzyme AOE372; AOE37-2; Peroxiredoxin IV; Prx-IV; Thioredoxin peroxidase AO372; Thioredoxin-dependent peroxide reductase A0372

PRDX4

PRDX4; PRDX4; PRX-4; AOE372; AOE37-2; HEL-S-97n; AOE37-2; Prx-IV

PRDX4_HUMAN

Human

271

This assay has high sensitivity and excellent specificity for detection of Human PRDX4. No significant cross-reactivity or interference between Human PRDX4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PRDX4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for PRDX4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PRDX4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PRDX4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

5453549

NP_006397.1

NM_006406.1

Q13162

30,540 Da

Selenium Pathway (198825)

The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB. [provided by RefSeq, Jul 2008]

Function: Probably involved in redox regulation of the cell. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.1 PublicationManual assertion based on experiment in:Ref.1. Catalytic activity: 2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150