Human iduronate sulfatase, IDS ELISA Kit | MyBioSource MBS705044 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human iduronate sulfatase, IDS ELISA Kit

catalog :

MBS705044

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705044

products type :

ELISA Kit

products full name :

Human iduronate sulfatase, IDS ELISA Kit

products short name :

iduronate 2-sulfatase

products name syn :

Human iduronate sulfatase; IDS ELISA Kit; MPS2; SIDS; alpha-L-iduronate sulfate sulfatase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; iduronate-2-sulfatase; idursulfase; iduronate 2-sulfatase

other names :

iduronate 2-sulfatase isoform a preproprotein; Iduronate 2-sulfatase; iduronate 2-sulfatase; idursulfase; iduronate 2-sulfatase 14 kDa chain; iduronate 2-sulfatase 42 kDa chain; alpha-L-iduronate sulfate sulfatase; iduronate 2-sulfatase; Alpha-L-iduronate sulfate sulfatase; Idursulfase

products gene name :

IDS

other gene names :

IDS; IDS; MPS2; SIDS; SIDS; Idursulfase

uniprot entry name :

IDS_HUMAN

reactivity :

Human

sequence length :

550

specificity :

This assay has high sensitivity and excellent specificity for detection of human IDS. No significant cross-reactivity or interference between human IDS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human IDS is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IDS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IDS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IDS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557659

ncbi acc num :

NP_000193.1

ncbi gb acc num :

NM_000202.6

uniprot acc num :

P22304

ncbi mol weight :

61,873 Da

ncbi pathways :

CS/DS Degradation Pathway (645311); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Dermatan Sulfate Degradation Pathway (413375); Dermatan Sulfate Degradation Pathway (468240); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Glycosaminoglycan Degradation Pathway (82981); Glycosaminoglycan Degradation Pathway (355); Glycosaminoglycan Metabolism Pathway (645297); HS-GAG Degradation Pathway (645307)

ncbi summary :

The protein encoded by this gene belongs to the sulfatase family, is localized to the lysosome, and is involved in lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations in this gene are associated with the X-linked lysosomal storage disease, mucopolysaccharidosis type II, also known as Hunter syndrome. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Aug 2013]

uniprot summary :

Function: Required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Catalytic activity: Hydrolysis of the 2-sulfate groups of the L-iduronate 2-sulfate units of dermatan sulfate, heparan sulfate and heparin. Cofactor: Binds 1 calcium ion per subunit

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage