ELISA/assay

Human Collagen Type II, Col II ELISA Kit

MBS705041

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Collagen Type II, Col II ELISA Kit

[Collagen Type II, Col II]

[Human Collagen Type II; Col II ELISA Kit; Collagen Type II; Col II]

[Col II]

Human

This assay has high sensitivity and excellent specificity for detection of human CNDP1. No significant cross-reactivity or interference between human CNDP1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.04 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CNDP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CNDP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CNDP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CNDP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465