Rat matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit | MyBioSource MBS705039 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

catalog :

MBS705039

quantity :

48-Strip-Wells

price :

465 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705039

products type :

ELISA Kit

products full name :

Rat matrix metalloproteinase 2/Gelatinase A, MMP-2 ELISA Kit

products short name :

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

products name syn :

Rat matrix metalloproteinase 2/Gelatinase A; MMP-2 ELISA kit; CLG4; CLG4A; MMP-II; MONA; TBE-1; collagenase type IV-A; matrix metalloproteinase 2; matrix metalloproteinase-II; neutrophil gelatinase; matrix metallopeptidase 2 (gelatinase A; 72kDa gelatinase; 72kDa type IV collagenase)

other names :

72 kDa type IV collagenase; 72 kDa type IV collagenase; 72 kDa type IV collagenase; MMP-2; gelatinase A; 72 kDa gelatinase; matrix metalloproteinase-2; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; MMP-2

products gene name :

MMP2

other gene names :

MMP2; MMP2; MMP-2

uniprot entry name :

MMP2_RABIT

reactivity :

Rat

sequence length :

662

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat MMP2. No significant cross-reactivity or interference between Rat MMP2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MMP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain MMP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

ncbi gi num :

126722977

ncbi acc num :

NP_001075678.1

ncbi gb acc num :

NM_001082209.1

uniprot acc num :

P50757

ncbi mol weight :

73,803 Da

ncbi pathways :

Bladder Cancer Pathway (83503); Bladder Cancer Pathway (527); GnRH Signaling Pathway (83483); GnRH Signaling Pathway (502); IL-3 Signaling Pathway (198504); Leukocyte Transendothelial Migration Pathway (83475); Leukocyte Transendothelial Migration Pathway (494); Matrix Metalloproteinases Pathway (198492); Pathways In Cancer (83493)

uniprot summary :

Function: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Involved in the formation of the fibrovascular tissues . Catalytic activity: Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly- -Ile-Ala-Gly-Gln. Cofactor: Binds 4 calcium ions per subunit

size1 :

48-Strip-Wells

price1 :

465 USD

size2 :

96-Strip-Wells

price2 :

655

size3 :

5x96-Strip-Wells

price3 :

2455

size4 :

10x96-Strip-Wells

price4 :

4595

more info or order :

MyBioSource product webpage