catalog number :
MBS705030
products type :
ELISA Kit
products full name :
Mouse beta-defensins 1 ELISA Kit
products short name :
defensin, beta 1
products name syn :
Mouse beta-defensins 1 ELISA Kit; BD1; DEFB-1; DEFB101; HBD1; MGC51822; beta-defensin-1; defensin; beta 1
other names :
beta-defensin 1; Beta-defensin 1; beta-defensin 1; defensin beta 1; Defensin, beta 1
products gene name :
DEFB1
other gene names :
Defb1; Defb1; BD-1; AW260221; BD-1; mBD-1
uniprot entry name :
DEFB1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse beta-defensins 1. No significant cross-reactivity or interference between mouse beta-defensins 1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-defensins 1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-defensins 1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-defensins 1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-defensins 1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_031869.1
ncbi gb acc num :
NM_007843.3
ncbi mol weight :
7,749 Da
ncbi pathways :
Beta Defensins Pathway (1001262); Defensins Pathway (1001260); Immune System Pathway (971216); Innate Immune System Pathway (971212)
uniprot summary :
Function: Has bactericidal activity
size4 :
10x96-Strip-Wells