TGIF1 Antibody | MyBioSource MBS7050265 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

TGIF1 Antibody

catalog :

MBS7050265

quantity :

0.05 mg

price :

160 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, enzyme immunoassay

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot. Positive WB detected in:Mouse heart tissue,Mouse liver tissue,Mouse kidney tissue. All lanes: TGIF1 antibody at 3ug/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 44,30,32,28 kDa. Observed band size: 44,50 kDa.

image 2 :

Immunohistochemistry of paraffin-embedded human testis tissue using MBS7050265 at dilution of 1:100

image 3 :

Immunofluorescent analysis of Hela cells using MBS7050265 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

product information

catalog number :

MBS7050265

products type :

Antibody

products full name :

TGIF1 Antibody

products short name :

[TGIF1]

products name syn :

[Homeobox protein TGIF1; 5'-TG-3'-interacting factor 1; TGIF1; TGIF]

other names :

[homeobox protein TGIF1 isoform e; Homeobox protein TGIF1; homeobox protein TGIF1; TGFB induced factor homeobox 1; 5'-TG-3'-interacting factor 1]

products gene name :

[TGIF1]

other gene names :

[TGIF1; TGIF1; HPE4; TGIF; TGIF]

uniprot entry name :

TGIF1_HUMAN

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

reactivity :

Human, Mouse

sequence length :

275

purity :

>95%, Protein G purified

form :

Liquid

storage stability :

Upon receipt, store at -20°C or -80°C. Avoid freeze/thaw.

tested application :

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

app notes :

WB: 1: 500-1: 5000. IHC: 1: 20-1: 200. IF: 1: 50-1: 200

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Immunogen: Recombinant Human Homeobox protein TGIF1 protein (8-375AA). Species: Human. Research Area: Neuroscience

other info2 :

Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. Conjugation: Non-conjugated. Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-9084 / sc-9825

products description :

Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.

ncbi gi num :

522838237

ncbi acc num :

NP_001265611.1

ncbi gb acc num :

NM_001278682.1

uniprot acc num :

Q15583

ncbi pathways :

Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); Downregulation Of SMAD2/3:SMAD4 Transcriptional Activity Pathway (1269529); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Regulation Of Nuclear SMAD2/3 Signaling Pathway (137963); SMAD2/SMAD3:SMAD4 Heterotrimer Regulates Transcription Pathway (1269528); Signal Transduction Pathway (1269379); Signaling By TGF-beta Receptor Complex Pathway (1269523); TGF-beta Receptor Signaling Pathway (198810)

ncbi summary :

The protein encoded by this gene is a member of the three-amino acid loop extension (TALE) superclass of atypical homeodomains. TALE homeobox proteins are highly conserved transcription regulators. This particular homeodomain binds to a previously characterized retinoid X receptor responsive element from the cellular retinol-binding protein II promoter. In addition to its role in inhibiting 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element, the protein is an active transcriptional co-repressor of SMAD2 and may participate in the transmission of nuclear signals during development and in the adult. Mutations in this gene are associated with holoprosencephaly type 4, which is a structural anomaly of the brain. Alternative splicing has been observed at this locus and multiple splice variants encoding distinct isoforms are described. [provided by RefSeq, Jul 2013]

uniprot summary :

TGIF: Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII- RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities. Defects in TGIF1 are the cause of holoprosencephaly type 4 (HPE4). Holoprosencephaly (HPE) is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. Belongs to the TALE/TGIF homeobox family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription, coactivator/corepressor. Chromosomal Location of Human Ortholog: 18p11.3. Cellular Component: nucleoplasm. Molecular Function: protein binding; transcription corepressor activity; transcription factor activity. Biological Process: multicellular organismal development; negative regulation of transcription from RNA polymerase II promoter. Disease: Holoprosencephaly 4

size1 :

0.05 mg

price1 :

160 USD

size2 :

0.1 mg

price2 :

235

more info or order :

MyBioSource product webpage