Human milk fat globule-EGF factor 8 protein, MFGE8 ELISA Kit | MyBioSource MBS705026 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human milk fat globule-EGF factor 8 protein, MFGE8 ELISA Kit

catalog :

MBS705026

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705026

products type :

ELISA Kit

products full name :

Human milk fat globule-EGF factor 8 protein, MFGE8 ELISA Kit

products short name :

milk fat globule-EGF factor 8 protein

products name syn :

Human milk fat globule-EGF factor 8 protein (MFGE8) ELISA kit; BA46; EDIL1; HsT19888; MFG-E8; OAcGD3S; SED1; SPAG10; hP47; O-acetyl disialoganglioside synthase; lactadherin; medin; sperm associated antigen 10; sperm surface protein hP47; milk fat globule-EGF factor 8 protein

other names :

lactadherin isoform b; Lactadherin; lactadherin; medin; sperm surface protein hP47; sperm associated antigen 10; breast epithelial antigen BA46; O-acetyl disialoganglioside synthase; milk fat globule-EGF factor 8 protein; Breast epithelial antigen BA46; HMFG; MFGM; Milk fat globule-EGF factor 8; MFG-E8; SED1Cleaved into the following 2 chains:Lactadherin short form; Medin

products gene name :

MFGE8

other gene names :

MFGE8; MFGE8; BA46; HMFG; MFGM; SED1; hP47; EDIL1; MFG-E8; SPAG10; OAcGD3S; HsT19888; MFG-E8

uniprot entry name :

MFGM_HUMAN

reactivity :

Human

sequence length :

335

specificity :

This assay has high sensitivity and excellent specificity for detection of Human MFGE8. No significant cross-reactivity or interference between Human MFGE8 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, cell culture supernates, urine, cerebrospinal fluid (CSF). Assay Type: Sandwich. Detection Range: 781.25 pg/ml-50000 pg/ml. Sensitivity: 195.3 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

For Sample: For the quantitative determination of human milk fat globule-EGF factor 8 protein (MFGE8) concentrations in serum, cell culture supernates, urine, cerebrospinal fluid (CSF). PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MFGE8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MFGE8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MFGE8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MFGE8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

167830477

ncbi acc num :

NP_001108086.1

ncbi gb acc num :

NM_001114614.1

uniprot acc num :

Q08431

ncbi mol weight :

43,123 Da

ncbi pathways :

Amyloids Pathway (366238); Disease Pathway (530764); Integrins In Angiogenesis Pathway (137999)

ncbi summary :

This gene encodes a preproprotein that is proteolytically processed to form multiple protein products. The major encoded protein product, lactadherin, is a membrane glycoprotein that promotes phagocytosis of apoptotic cells. This protein has also been implicated in wound healing, autoimmune disease, and cancer. Lactadherin can be further processed to form a smaller cleavage product, medin, which comprises the major protein component of aortic medial amyloid (AMA). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

uniprot summary :

Function: Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.1 PublicationManual assertion based on experiment in:Ref.15

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage