product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat KI-67 ELISA Kit
catalog :
MBS705024
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS705024
products type :
ELISA Kit
products full name :
Rat KI-67 ELISA Kit
products short name :
KI-67
products name syn :
Rat KI-67 ELISA kit; MKI67; KI-67
other names :
Ki-67; Ki-67 protein; Ki-67 protein
products gene name :
KI-67
other gene names :
Mki67; Ki-67
uniprot entry name :
Q61769_MOUSE
reactivity :
Rat
sequence length :
2938
specificity :
This assay has high sensitivity and excellent specificity for detection of rat KI-67. No significant cross-reactivity or interference between rat KI-67 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat KI-67 is typically less than 0.04 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KI-67 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KI-67 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KI-67 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KI-67 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
1177528
ncbi acc num :
CAA58026.1
uniprot acc num :
Q61769
ncbi mol weight :
324,429 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers. Protein type: Cell cycle regulation; Nucleolus. Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region. Molecular Function: protein C-terminus binding. Biological Process: cell proliferation; meiosis
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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