ELISA/assay

Human Mucin 2, MUC2 ELISA Kit

MBS705019

48-Strip-Wells

565 USD

ELISA Kit

Human Mucin 2, MUC2 ELISA Kit

mucin 2, oligomeric mucus/gel-forming

Human Mucin 2 (MUC2) ELISA Kit; MLP; SMUC; mucin 2; mucin 2; intestinal/tracheal; mucin-like protein; mucin 2; oligomeric mucus/gel-forming

mucin-2; Mucin-2; mucin-2; intestinal mucin-2; mucin-like protein; mucin 2, intestinal/tracheal; mucin 2, oligomeric mucus/gel-forming; Intestinal mucin-2

MUC2

MUC2; MUC2; MLP; SMUC; MUC-2; SMUC; MUC-2

MUC2_HUMAN

Human

5179

This assay has high sensitivity and excellent specificity for detection of human MUC2. No significant cross-reactivity or interference between human MUC2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma and cell culture supernates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.78 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

557129045

NP_002448.3

NM_002457.3

Q02817

540,300 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748); Vibrio Cholerae Infection Pathway (83101); Vibrio Cholerae Infection Pathway (513)

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Function: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.1 PublicationManual assertion based on experiment in:Ref.11

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150