ELISA/assay

Human alpha-L-iduronidase, IDUA ELISA Kit

MBS705013

96 Strip Wells

850 USD

ELISA Kit

Human alpha-L-iduronidase, IDUA ELISA Kit

iduronidase, alpha-L-

Human alpha-L-iduronidase; IDUA ELISA Kit; IDA; MPS1; alpha-L-iduronidase; iduronidase; alpha-L-

alpha-L-iduronidase; Alpha-L-iduronidase; alpha-L-iduronidase; iduronidase, alpha-L-

IDUA

IDUA; IDUA; IDA; MPS1

IDUA_HUMAN

Human

653

This assay has high sensitivity and excellent specificity for detection of Human IDUA. No significant cross-reactivity or interference between Human IDUA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell lysates and tissue homogenates. Detection Range: 0.156 ng/ml-10 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.039 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IDUA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IDUA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IDUA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IDUA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

110611239

NP_000194.2

NM_000203.4

P35475

72,670 Da

CS/DS Degradation Pathway 645311!!Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway 645308!!Dermatan Sulfate Degradation Pathway 413375!!Dermatan Sulfate Degradation Pathway 468240!!Disease Pathway 530764!!Glycogen Storage Diseases Pathway 980468!!Glycosaminoglycan Degradation Pathway 82981!!Glycosaminoglycan Degradation Pathway 355!!Glycosaminoglycan Metabolism Pathway 645297!!HS-GAG Degradation Pathway 645307

This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008]

96 Strip Wells

850 USD