Mouse matrix metalloproteinase 13, MMP-13 ELISA Kit | MyBioSource MBS705012 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse matrix metalloproteinase 13, MMP-13 ELISA Kit

catalog :

MBS705012

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705012

products type :

ELISA Kit

products full name :

Mouse matrix metalloproteinase 13, MMP-13 ELISA Kit

products short name :

matrix metallopeptidase 13 (collagenase 3)

products name syn :

Mouse matrix metalloproteinase 13; MMP-13 ELISA kit; CLG3; MANDP1; collagenase 3; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3) ; matrix metallopeptidase 13 (collagenase 3)

other names :

collagenase 3 preproprotein; Collagenase 3; collagenase 3; Collagenase-3; collagenase-1; interstitial collagenase; matrix metalloproteinase 13; matrix metalloproteinase-13; matrix metallopeptidase 13; Matrix metalloproteinase-13; MMP-13

products gene name :

MMP13

other gene names :

Mmp13; Mmp13; Clg; Mmp1; MMP-13; MMP-13

uniprot entry name :

MMP13_MOUSE

reactivity :

Mouse

sequence length :

472

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse MMP-13. No significant cross-reactivity or interference between mouse MMP-13 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 93.75 pg/ml -6000 pg/ml. Sensitivity: The minimum detectable dose of mouse MMP-13 is typically less than 23.43 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-13 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6678896

ncbi acc num :

NP_032633.1

ncbi gb acc num :

NM_008607.2

uniprot acc num :

P33435

ncbi mol weight :

54,182 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1001029); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1001018); Collagen Degradation Pathway (1001030); Collagen Formation Pathway (1001016); Degradation Of The Extracellular Matrix Pathway (1001028); Endochondral Ossification Pathway (198336); Extracellular Matrix Organization Pathway (1001015); Matrix Metalloproteinases Pathway (198370)

ncbi summary :

This gene encodes a member of the matrix metalloproteinase family that plays a role in wound healing, skeletal development and bone remodeling. The encoded protein is activated by the removal of an N-terminal activation peptide to generate a zinc-dependent endopeptidase enzyme that can cleave various native collagens, including types I - IV, X and XIV. Mice lacking the encoded protein display profound defects in growth plate cartilage as well as a delay in the endochondral bone development. Lack of the encoded protein also impairs the wound healing process due to reduced keratinocyte migration and vascular density at the wound site. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Jun 2015]

uniprot summary :

Function: Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.5 PublicationsManual assertion based on experiment in:Ref.2. Cofactor: Calcium. Can bind about 5 calcium ions per subunit.1 PublicationManual assertion based on experiment in:Ref.7

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage