Human sonic hedgehog N-Terminus, Shh-N ELISA Kit | MyBioSource MBS705010 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human sonic hedgehog N-Terminus, Shh-N ELISA Kit

catalog :

MBS705010

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705010

products type :

ELISA Kit

products full name :

Human sonic hedgehog N-Terminus, Shh-N ELISA Kit

products short name :

sonic hedgehog N-Terminus, Shh-N

products name syn :

Human sonic hedgehog N-Terminus; Shh-N ELISA Kit; sonic hedgehog N-Terminus; Shh-N

other names :

Sonic hedgehog protein; Sonic hedgehog protein; sonic hedgehog protein; sonic hedgehog homolog; sonic hedgehog; HHG-1Cleaved into the following 2 chains:Sonic hedgehog protein N-product; Sonic hedgehog protein C-product

products gene name :

Shh-N

other gene names :

SHH; SHH; TPT; HHG1; HLP3; HPE3; SMMCI; TPTPS; MCOPCB5; SHH

uniprot entry name :

SHH_HUMAN

reactivity :

Human

sequence length :

462

specificity :

This assay has high sensitivity and excellent specificity for detection of human Shh-N. No significant cross-reactivity or interference between human Shh-N and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 62. 5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of human Shh-N is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Shh-N has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Shh-N present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Shh-N is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Shh-N bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6094283

ncbi acc num :

Q15465.1

uniprot acc num :

Q15465

ncbi mol weight :

49,607 Da

ncbi pathways :

Basal Cell Carcinoma Pathway (83113); Basal Cell Carcinoma Pathway (525); Class B/2 (Secretin Family Receptors) Pathway (106378); Disease Pathway (530764); FOXA1 Transcription Factor Network Pathway (137979); GPCR Ligand Binding Pathway (161020); Glypican 3 Network Pathway (138084); Heart Development Pathway (198802); Hedgehog Signaling Pathway (198835); Hedgehog Ligand Biogenesis Pathway (1016168)

ncbi summary :

This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO 1 PublicationManual assertion based on experiment in:Ref.11

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage