ELISA/assay

Human Ischemia Modified Albumin, IMA ELISA Kit

MBS705008

96 Strip Wells

850 USD

ELISA Kit

Human Ischemia Modified Albumin, IMA ELISA Kit

Ischemia Modified Albumin, IMA

Human Ischemia Modified Albumin; IMA ELISA Kit; Ischemia Modified Albumin; IMA

IMA

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Sample Type: serum, plasma, tissue homogenates. Detection Range: 3.12 IU/ml-200 IU/ml. Detection Wavelength: 450 nm. Sensitivity: 0.78 IU/ml

Sample Volume: 50-100ul

Introduction: Ischemia-modified albumin (IMA), a new marker of myocardial ischemia, is known to elevate during ischemia induced by percutaneous coronary intervention. It is, however, not known whether ischemia-modified albumin also elevates during transient coronary vasospasm. IMA is highly sensitive for the diagnosis of myocardial ischaemia in patients presenting with symptoms of acute chest pain. IMA is also a good alternative to D-dimer in the exclusion of PE diagnosis in terms of cost and efficiency. When used in combination with clinical probability scores, it has a similar positive effect on sensitivity to that of D-dimer and a superior one to that of NPV. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IMA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IMA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

96 Strip Wells

850 USD